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1月25日

Carbohydrates

Carbohydrates
A carbohydrate is an organic compound that is composed of atoms of carbon, hydrogen and oxygen in a ratio of 1 carbon atom, 2 hydrogen atoms, and 1 oxygen atom. Some carbohydrates are relatively small molecules, the most important to us is glucose which has 6 carbon atoms. These simple sugars are called monosaccharides.

The primary function of carbohydrates is for short-term energy storage (sugars are for Energy). A secondary function is intermediate-term energy storage (as in starch for plants and glycogen for animals). Other carbohydrates are involved as structural components in cells, such as cellulose which is found in the cell walls of plants.

star Two common Monosaccharides, (single sugars) Glucose and Fructose

Hooking two monosaccharides together forms a more complex sugar, such as the union of glucose and fructose to give sucrose, or common table sugar. Compounds such as sucrose are called Disaccharides (two sugars). Both monosaccharides and disaccharides are soluble in water.

Larger, more complex carbohydrates are formed by linking shorter units together to form long or very long sugar chains called Polysaccharides. Because of their size, these are often times not soluble in water. Many biologically important compounds such as starches and cellulose are Polysaccharides. Starches are used by plants, and glycogen by animals, to store energy in their numerous carbon-hydrogen bonds, while cellulose is an important compound that adds strength and stiffness to a plant's cell wall.

Sugars are most often found in the form of a "RING". The glucose molecule in the image above and the one in the image below (Glc) are really the same molecule, just arranged differently. The corners of the "stop sign" represent Carbon atoms even thought they are not labeled with a "C" (its chemistry shorthand). To form these rings, the Carbonyl (C=0) Carbon of the straight-chain form (above) forms a bond with the next to last Carbon in the chain, making the ring.

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The image on the left shows two monosaccharides, Glucose and Galactose (Gal). Examine their structure and you will notice there is very little difference. Their molecular formulas, C6H1206, are even the same. Molecules with the same chemical formula, but different molecular structures are called Isomers.

The sugar subunits can be linked by the reaction, dehydration synthesis, to form larger molecules. The disaccharide, Sucrose, is formed from two monosaccharides, Glucose and Fructose.

The disaccharide Lactose is a dimer (two subunits) of Glucose and Galactose, the disaccharide Maltose is a dimer of Glucose.

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Large polymers of sugars are called Carbohydrates. Carbohydrates can be 100's of sugars long and either straight or branched. The term Complex Carbohydrate, or sometimes even just Carbohydrate refers to long chains of sugars. Three common types of complex carbo's we will examine are: Starch, Cellulose, and Glycogen. All three are composed only of Glucose. They differ only in the bonding arrangements between the Glucose subunits. Not all complex carbs are composed of glucose alone, many have highly unusual sugars in their chains.

Starch is a long (100's) polymer of Glucose molecules, where all the sugars are oriented in the same direction. Starch is one of the primary sources of calories for humans.

Cellulose is a long (100's) polymer of Glucose molecules. However the orientation of the sugars is a little different. In Cellulose, every other sugar molecule is "upside-down". This small difference in structure makes a big difference in the way we use this molecule.

Glycogen is another Glucose polymer. Glycogen is a stored energy source, found in the Liver and muscles of Humans. Glycogen is different from both Starch and Cellulose in that the Glucose chain is branched or "forked".

1月19日

Anatomy of cells

 

There are two types of cells, eukaryotic and prokaryotic. Prokaryotic cells are usually singletons, while eukaryotic cells are usually found in multi-cellular organisms.

Prokaryotic cells

 

A typical prokaryotic cell
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A typical prokaryotic cell

Prokaryotes are distinguished from eukaryotes on the basis of nuclear organization, specifically their lack of a nuclear membrane. Prokaryotes also lack most of the intracellular organelles and structures that are characteristic of eukaryotic cells (an important exception is the ribosomes, which are present in both prokaryotic and eukaryotic cells). Most of the functions of organelles, such as mitochondria, chloroplasts, and the Golgi apparatus, are taken over by the prokaryotic plasma membrane. Prokaryotic cells have three architectural regions: appendages called flagella and pili — proteins attached to the cell surface; a cell envelope consisting of a capsule, a cell wall, and a plasma membrane; and a cytoplasmic region that contains the cell genome (DNA) and ribosomes and various sorts of inclusions. Other differences include:

  • The plasma membrane (a phospholipid bilayer) separates the interior of the cell from its environment and serves as a filter and communications beacon.
  • Most prokaryotes have a cell wall (some exceptions are Mycoplasma (a bacterium) and Thermoplasma (an archaeon)). It consists of peptidoglycan in bacteria, and acts as an additional barrier against exterior forces. It also prevents the cell from "exploding" (cytolysis) from osmotic pressure against a hypotonic environment. A cell wall is also present in some eukaryotes like fungi, but has a different chemical composition.
  • A prokaryotic chromosome is usually a circular molecule (an exception is that of the bacterium Borrelia burgdorferi, which causes Lyme disease). Even without a real nucleus, the DNA is condensed in a nucleoid. Prokaryotes can carry extrachromosomal DNA elements called plasmids, which are usually circular. Plasmids can carry additional functions, such as antibiotic resistance.

Eukaryotic cells

A typical eukaryotic cell
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A typical eukaryotic cell

Eukaryotic cells are about 10 times the size of a typical prokaryote and can be as much as 1000 times greater in volume. The major difference between prokaryotes and eukaryotes is that eukaryotic cells contain membrane-bound compartments in which specific metabolic activities take place. Most important among these is the presence of a cell nucleus, a membrane-delineated compartment that houses the eukaryotic cell's DNA. It is this nucleus that gives the eukaryote its name, which means "true nucleus". Other differences include:

  • The plasma membrane resembles that of prokaryotes in function, with minor differences in the setup. Cell walls may or may not be present.
  • The eukaryotic DNA is organized in one or more linear molecules, called chromosomes, which are associated with histone proteins. All chromosomal DNA is stored in the cell nucleus, separated from the cytoplasm by a membrane. Some eukaryotic organelles also contain some DNA.
  • Eukaryotes can move using cilia or flagella. The flagella are more complex than those of prokaryotes.
Table 1: Comparison of features of prokaroytic and eukaryotic cells
  Prokaryotes Eukaryotes
Typical organisms bacteria, archaea protists, fungi, plants, animals
Typical size ~ 1-10 µm ~ 10-100 µm (sperm cells, apart from the tail, are smaller)
Type of nucleus nucleoid region; no real nucleus real nucleus with double membrane
DNA circular (usually) linear molecules (chromosomes) with histone proteins
RNA-/protein-synthesis coupled in cytoplasm RNA-synthesis inside the nucleus
protein synthesis in cytoplasm
Ribosomes 50S+30S 60S+40S
Cytoplasmatic structure very few structures highly structured by endomembranes and a cytoskeleton
Cell movement flagella made of flagellin flagella and cilia made of tubulin
Mitochondria none one to several dozen (though some lack mitochondria)
Chloroplasts none in algae and plants
Organization usually single cells single cells, colonies, higher multicellular organisms with specialized cells
Cell division Binary fission (simple division) Mitosis (fission or budding)
Meiosis
Table 2: Comparison of structures between animal and plant cells
Typical animal cell Typical plant cell
Organelles
Additional structures

Subcellular components

The cells of eukaryotes (left) and prokaryotes (right).
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The cells of eukaryotes (left) and prokaryotes (right).

All cells, whether prokaryotic or eukaryotic, have a membrane, which envelopes the cell, separates its interior from its environment, regulates what moves in and out (selectively permeable), and maintains the electric potential of the cell. Inside the membrane, a salty cytoplasm takes up most of the cell volume. All cells possess DNA, the hereditary material of genes, and RNA, containing the information necessary to build various proteins such as enzymes, the cell's primary machinery. There are also other kinds of biomolecules in cells.

The Cell & Its Properties

 
Drawing of the structure of cork as it appeared under the microscope to Robert Hook from Micrographia which is the origin of the word "cell".
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Drawing of the structure of cork as it appeared under the microscope to Robert Hook from Micrographia which is the origin of the word "cell".
Cells in culture, stained for keratin (red) and DNA (green).
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Cells in culture, stained for keratin (red) and DNA (green).

The cell is the structural and functional unit of all living organisms, and is sometimes called the "building block of life."Some organisms, such as bacteria, are unicellular, consisting of a single cell. Other organisms, such as humans, are multicellular, (humans have an estimated 100 trillion or 1014 cells; a typical cell size is 10 µm, a typical cell mass 1 nanogram). The largest known cell is an ostrich egg.

The cell theory, first developed in 1839 by Schleiden and Schwann, states that all organisms are composed of one or more cells; all cells come from preexisting cells; all vital functions of an organism occur within cells, and cells contain the hereditary information necessary for regulating cell functions and for transmitting information to the next generation of cells.

The word cell comes from the Latin cellula, a small room. The name was chosen by Robert Hooke when he compared the cork cells he saw to the small rooms monks lived in.

Properties of cells

Each cell is at least somewhat self-contained and self-maintaining: it can take in nutrients, convert these nutrients into energy, carry out specialized functions, and reproduce as necessary. Each cell stores its own set of instructions for carrying out each of these activities.

Mouse cells grown in a culture dish. These cells grow in large clumps, but each individual cell is about 10 micrometres across.
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Mouse cells grown in a culture dish. These cells grow in large clumps, but each individual cell is about 10 micrometres across.

All cells share several abilities:

Some prokaryotic cells contain important internal membrane-bound compartments, but eukaryotic cells have a highly specialized endomembrane system characterized by regulated traffic and transport of vesicles.